2.b. Overview

The EchoMRI produces the following measurements:

  1. Fat mass
  2. Lean mass
  3. Total water
  4. Free water

These are recorded as absolute masses in grams, but I have also converted them to percentage of body mass for an additional way to assess body composition.

Similar to the metabolic tests, each mouse was measured at baseline and endpoint. Therefore, we can assess the effects of hormone treatment via:


2.b.i. Intragroup Longitudinal Changes in Body Comp

Longitudinal changes can be visually represented in a few dif ways:

Lean mass (g)

NOTES:

  • Because the mice are getting older, we might expect absolute lean mass to increase across the board. That’s why looking at % can be useful too.

  • There are some outliers I could consider taking out. I didn’t for now…

Lean mass (%)

Fat mass (g)

Fat mass (%)

Total water (g)

Total water (%)

Free water (g)

Free water (%)


2.b.ii. Endpoint Body Comp in each Hormone Group vs Saline

Lean mass (g)

Lean mass (%)

Fat mass (g)

Fat mass (%)

Total water (g)

Total water (%)

Free water (g)

Free water (%)


2.b.iii. Endpoint Body Comp in each Hormone Group ABX vs Non-ABX

Lean mass (g)

Lean mass (%)

Fat mass (g)

Fat mass (%)

Total water (g)

Total water (%)

Free water (g)

Free water (%)


2.b.iv. Correlations of Body Comp with other Factors

Correlations with body weight

Does the relationship between these variables and total body weight differ by ABX status? No clear differences in relationship by ABX status.

Let’s plot absolute lean mass (g) vs absolute body weight (g) in each group.

To see if there are differences in this relationship between hormone groups, we will have to filter to only include the endpoint values. This means we will be working with very low sample sizes for a regression, so these are probably going to be not informative graphs. But nonetheless here they are!

How about lean mass percentage vs absolute body weight (g)?

Let’s plot absolute fat mass (g) vs absolute body weight (g)

How about free mass percentage vs absolute body weight (g)? Not that interesting.